Biological activity of a drug, polypeptides derived from testes in a model of oxidative stress in vitro.

Introduction. Male infertility is one of the main causes forced childlessness couples around world. Currently, a limited number drugs for treatment male are available on market. Biologically active supplements (dietary supplements) containing L-carnitine often used in complex therapy. The results determining therapeutic activity new drug, peptide obtained from cattle testes (PP-R-001) and comparing its effectiveness with models oxidative stress induction cell cultures presented. Material methods. study was carried out lines transplanted piglet testicle (PTP) bovine embryonic (TEB). Two experimental approaches were to induce (OS) testicular cells: co-culturing cells either PTP or TEB cisplatin (cis-Pt) hydrogen peroxide (H2O2). protective effect PP-R-001 assessed by apoptosis death, migration activity, DNA double-strand breaks, level reactive oxygen species, mitochondrial under OS conditions. Results. significantly exceeded survival rate decrease apoptotic increased both lines. It found that reduced intracellular lesions lead death exposed cis-Pt H2O2: drug mitochondria decreased breaks species (ROS). Conclusion. data indicate has multimodal models, which superior widely fertility supplements.